Identification of the covalently bound flavin prosthetic group of cholesterol oxidase.
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چکیده
منابع مشابه
Covalently bound flavin as prosthetic group of choline oxidase.
loxiazine ring. Effect of pH on fluorescence of the flavin peptide was shown in fig. 1. The fluorescence of the flavin peptide was quenched at neutral and alkaline pH ranges. This shows that the amino acid linked to flavin ring is a histidyl residue.6) The pKa. value of the flavin peptide was found to be about 4.6, which was almost identical with that of the authentic 8a-[7V(3)-histidyl]ribofla...
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Following elucidation of the structures of the flavin components of succinate dehydrogenase (SD) as N (3)-histidyl-8a-FAD and of monoamine oxidase (MAO) as cysteinyl-8a-FAD and determination of the peptide sequences around the flavin sites of these enzymes, attention has been focused on the covalently bound FAD of Chromatium cytochrome c-552. As documented in preliminary communications, the FAD...
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Dimethylglycine dehydrogenase (EC 1.5.99.2) and sarcosine dehydrogenase (EC 1.5.99.1) are the folate binding proteins of rat liver mitochondria. These two enzymes contain covalently bound flavin and catalyze similar oxidative demethylation reactions (Wittwer, A. J., and Wagner, C. (1981) J. Biol. Chem. 256, 4102-4108). Flavin-peptides have been purified from these two enzymes after proteolytic ...
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oxidase 3 . Moreover, methylene blue was shown to be a strong inhibitor of the overall reaction. Oneelectron acceptors are also ineffective with the Dspecific enzyme; however, methylene blue and 2.6dichlorophenol-indophenol are able to reoxidize the reduced D-6-hydroxynicotine oxidase. In the presence of these dyes the rates of the overall process are higher than those with oxygen. Anaerobic ti...
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This investigation was carried out at the Indian Institute of Science, Bangalore, where spectroscope facilities were available. The author is indebted to Sir J. C. Ghosh, Director, Indian Institute of Science, Bangalowe, for laboratory accommodation and to Mr M. Srinivasaya, Head of the Fermentation Technology Section at the Institute, for much helpful advice and discussion. Thanks are also due...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1979
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)30066-2